Anti-tumor and immunomodulatory activities of a novel polysaccharide from Grifola frondosa prepared by hydrogen peroxide/vitamin C-assisted extraction

文献标题：Anti-tumor and immunomodulatory activities of a novel polysaccharide from Grifola frondosa prepared by hydrogen peroxide/vitamin C-assisted extraction

标题翻译：过氧化氢/维⽣素C辅助提取制备的灰树花新型多糖的抗 肿瘤和免疫调节活性

作       者： Cheng Wan, Ying-Ying Xu, Liang Chen, Weiming Liu, Xingli Wang, Qing Gu & Tao Zhou

关  键  词： Grifola frondosa polysaccharide · Cell apoptosis · H1975 cells · Anti-tumor effect · Immunomodulatory activity 

科研机构：

1  Key Laboratory for Food Microbial Technology of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou 310018, Zhejiang, P. R. China 

2  Zhejiang Biosan Biotech Co., Ltd., No.116 Dayuan Street, Shuige Industrial Zone, Liandu, Lishui 323010, Zhejiang, P.R. China 

发表期刊：Journal of Food Measurement and Characterization (2024) 18:7402–7417 

摘         要： Polysaccharides derived Grifola frondosa (GFP) possess diverse biological activities. In this study, a GFP fraction (coded as GFP1) purified from the crude GFP prepared by an H2O2/Vc-assisted extraction method was investigated for its inhibi tory effects on lung cancer cells (H1975) and immunomodulatory activities in RAW264.7 macrophages. It was found that GFP1 significantly inhibited the proliferation, induced the cell apoptosis, and suppressed the migration and invasion of H1975 cells. Additionally, GFP1 increased the intracellular reactive oxygen species (ROS) levels, reduced mitochondrial membrane potential, and upregulated the expressions of caspase-3, caspase-8, and caspase-9. RT-qPCR analysis indicated that GFP1 downregulated the mRNA expression levels of PI3K, ERK2, ERK1, FAK, and RAF. GFP1 was also found to upregulate the mRNA expressions of nitric oxide synthase (iNOS), IL-1β, IL-6, and TNF-α, thereby promoting the release of nitric oxide and secretion of the corresponding cytokines. Western blot experiments indicated that GFP1 activated the NF-κB signaling pathway through the promotion of p65 phosphorylation, exerting immunomodulatory effects. Therefore, GFP1 exerted anti-tumor effect by both direct cytotoxic effects on tumor cells and modulating the body’s immune func tion. These findings provide new insights for the development of functional foods and effective and safe anti-tumor drugs.

参考资料：https://link.springer.com/article/10.1007/s11694-024-02736-y