Effects of Simulated Digestion on the Structureand Macrophages and Dendritic Cells Activation of Grifola frondosa Polysaccharides

信息来源：互联网发布日期：2025年07月09日

摘要：The present work was analyzed the effects of simulated digestion on the structure of a multibranched polysaccharide (PM) from Grifola frondosa. Additionally, the activation effect of PM on macrophages and dendritic cells was compared before and after simulated digestion. The PM was composed of glucose, glucuronic acid, xylose, mannose, arabinose, and galactose in molar ratios of 81.6:6.1:5.4:3.5:2.5:1.0, with an average molecular weight of 3.04×10⁴ g/mol. The PM main chain is alternately linked by →3-β-D-Glcp-1→, →3-β-D-GlcpA-1→, →6-β-D-Glcp-1→, and →3,6)-β-D-Glcp-(1→, accompanied by multiple branched chains. PM significantly promotes macrophage proliferation, phagocytosis, and secretion of IL-6, IL-1β, and TNF-α, while reducing dendritic cell phagocytosis. During digestion, the branched chains linked with →3,4)-β-D-Xylp-(1→ and →3,4)-α-D-Manp-(1→ were mainly hydrolyzed in the gastric phase, while the glycosidic bonds and linkage sequences composing the main chain were not significantly changed. Meanwhile, the digested PM showed a slight enhancement in macrophages and dendritic cell activation. These results provide insight into the structural and immunoreactivity changes in G. frondosa polysaccharides after digestion and their potential role in intestinal immunity through activation of macrophages and dendritic cells.

文献标题：Effects of Simulated Digestion on the Structureand Macrophages and Dendritic Cells Activation of Grifola frondosa Polysaccharides

标题翻译：模拟消化对灰树花多糖结构及巨噬细胞和树突状细胞激活的影响

作者： Xiaoyan Chen，Xiong He，Shengwei Wang，Baoying Chen，Yanxian Feng， Ruili Yang，Chuanlun Yang，Jucai Xu，Congfa Li，Wu Li

关键词：
simulated digestion， Grifolafrondosa polysaccharides，structure characterization，macrophages，dendritic cells

科研机构： 1. Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China

2. Key Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou, China

3. Shandong Chambroad Holding Group Co., Ltd, Binzhou, China

4. College of Food Science, South China Agricultural University, Guangzhou, China

发表期刊：Journal of Food Science,2025;90:e70244

参考文献： https://doi.org/10.1111/1750-3841.70244

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