Synergisticimmuno-modulatory activity inhuman macrophages of a medicinalmushroom formulation consisting of Reishi.Shiitake and Maitake

文献标题：
Synergisticimmuno-modulatory activity inhuman macrophages of a medicinalmushroom formulation consisting of Reishi.Shiitake and Maitake

标题翻译：

由灵芝、⾹菇和灰树花组成的药⽤蘑菇由灵芝、⾹菇和灰树花组成的药⽤蘑菇 配方对人类巨噬细胞的协同免疫调节活配方对人类巨噬细胞的协同免疫调节活性

作         者：
BrodyMallard1,DavidN.Leach2,3,HansWohlmuth2,3,4,JoeTiralongoID1*

科研机构：

1 institute for Glycomics, Griffith University, Gold Coast, Queensland, Austraia,

2 integria Healthcare, EightMile Plains, Queensland, Australia,

3 National Institute of Complementary Medicine, Western Sydney University, Penrith, New South Wales, Australia,

4 School of Chemistry & Molecular Biosciences, The University of Queensland,St Lucia,Queensland, Australia

发表期刊：

PLOS ONE on November 7, 2019, with the DOI: 10.1371/journal.pone.0224740.

摘         要：

A key characteristic of mushroom polysaccharides that elicit an immunomodulatoryresponse is that they are rich in β-glucans and low in a-glucans. In this study we analysednine commercially available preparations from three mushroom species, Reishi (Gano.derma lucidum), Shitake (Lentinula edodes) and Maitake (Gnfola frondosa), for β- and a-glucan content. Based on B- and a-glucan content we selected three extracts to combineinto a fomula and evaluated the ability of the individual extracts and formula to impact onthe expression of cytokines lL-1a, lL-6, lL-10 and TNF-a in human macrophages with andwithout LPS stimulation. The majority of mushroom extracts and the formula were found tobe highly potentimmuno-stimulators possessing ECso values lower than 100 yg/mL. Inter-estingly the mushroom formula had lower ECso values in TNF-a expression from LPS stimu.lated macrophages compared to the individual extracts, suggesting a potential synergisticeffect of the mushroom formula. A response additivity graph and curve-shift analysis illus-trated that indeed the mushroom formula exhibited an immuno-stimulatory synergistic effecton the expression of the majority of cytokines evaluated in both LPS stimulated and non-stimulated human macrophages, with lL-10 having an antagonistic response. This studyrepresents the first report of a synergistic immuno-modulatory response in human macrophages elicited from a mushroom formula rationally derived from B- and a-glucan content.

参考文献：https://doi.org/10.1371/journal.pone.0224740